Standalone Assay Design Tool

Assay Stoichiometry Calculator

Estimate whether a nanoscale staining assay is most likely limited by accessible target abundance, physical packaging on the particle, or bulk probe amount per particle. This is the stoichiometry layer extracted from the Vesicle Surface Explorer and turned into a focused calculator.

Probe molecules per particle Geometry-only ceiling Likely main limiter

Particle

Context

Target regime

Surface distribution

Vesicle diameter

Surface accessible fraction

Accessibility here defines the surface patch that is truly available for binding. Higher particle curvature and clustered targets will reduce the geometry ceiling automatically.

Target

Supply

Target abundance input

Ligands per particle

count

Ligand accessible fraction

Binding-active fraction

Ligand footprint

Probe And Mix

Assay

Probe footprint

Steric buffer

Particle concentration

particles/mL

Probe concentration

Effective probe availability

Volume cancels out here. Probe concentration and particle concentration are compared within the same mixture to estimate the average probes available per particle.

Stoichiometry Outcome

Result

Accessible ligands 0

Geometry-only ceiling 0

Available probes per particle 0

                Effective final ceiling
                0
              

Likely main limiter n/a

Ceiling Comparison

Per particle

Accessible ligand supply 0

Geometry-only binding ceiling 0

Probe supply ceiling 0

Effective final ceiling 0

Key Ratios

Readout

Geometry retention n/a

Final retention n/a

Probe excess vs geometry n/a

Unoccupied accessible ligands n/a

Interpretation

Notes